A double-label pre-embedding immunoperoxidase technique for electron microscopy using diaminobenzidine and tetramethylbenzidine as markers.
نویسندگان
چکیده
منابع مشابه
An Embedding Technique for Electron Microscopy Using Epon 812
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Unambiguous identification of lymphocytes is sometimes difficult because of weak immunostaining of the cell membrane immunoglobulins. A simple method of intensifying the diaminobenzidine (DAB) peroxidase reaction was therefore devised. Paraffin wax sections of formalin fixed tonsils and lymphomas were digested with trypsin and immunostained for kappa and lambda light immunoglobulin chains and C...
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Epoxy resins are suitable media for embedding for electron microscopy, as they set uniformly with virtually no shrinkage. A mixture of araldite epoxy resins has been developed which is soluble in ethanol, and which yields a block of the required hardness for thin sectioning. The critical modifications to the conventional mixtures are the choice of a plasticized resin in conjunction with an alip...
متن کاملSilver enhancement-of polymerised diaminobenzidine: Increased sensitivity for immunoperoxidase staining
Unambiguous identification of lymphocytes is sometimes difficult because of weak immunostaining of the cell membrane immunoglobulins. A simple method of intensifying the diaminobenzidine (DAB) peroxidase reaction was therefore devised. Paraffin wax sections of formalin fixed tonsils and lymphomas were digested with trypsin and immunostained for K and A light immunoglobulin chains and CD3 antige...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1989
ISSN: 0022-1554,1551-5044
DOI: 10.1177/37.8.2666511